Effects of Lactobacillus rhamnosus DSM7133 on Intestinal Porcine Epithelial Cells

Antimicrobial resistance is one of the biggest health challenges nowadays. Probiotics are promising candidates as feed additives contributing to gastrointestinal tract. The beneficial effect probiotics species/strain specific; potential benefits need be individually assessed for each probiotic strain or species. We established a co-culture model, in which infection was modeled using Escherichia coli (E. coli) and Salmonella enterica serovar Typhimurium (S. Typhimurium). Using intestinal porcine epithelial cells (IPEC-J2), effects pre-, co-, post-treatment with Lactobacillus (L.) rhamnosus on barrier function, intracellular (IC) reactive oxygen species (ROS) production, proinflammatory cytokine (IL-6 IL-8) response, adhesion inhibition were tested. E. coli- S. Typhimurium-induced impairment increased ROS production could counteracted L. (p < 0.01). IL-6 reduced via pre-treatment 0.05) 0.01); IL-8 secretion decreased 0.01) rhamnosus. demonstrated significant both 0.001) 0.001 post-treatment; p 0.05 co-treatment). This study makes substantial contribution understanding specific Our findings can serve basis further vivo studies carried out pigs humans.